Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Cancer-associated fibroblast-derived circKLHL24 drives perineural invasion in pancreatic cancer via dual regulation of the sec31a-CXCL12 axis

doi: 10.1186/s13046-025-03489-2

Figure Lengend Snippet: circKLHL24/CXCL12 Axis Drives Perineural Invasion in Pancreatic Cancer In Vivo. ( A ) Gross and surgical images, H&E staining and mIF images. mIF showed CXCL12, FAP and Sec31A expression in the sciatic nerve invasion model. FAP served as a CAF marker. HE Scale bars, 1 mm. mIF Scale bars, 200 μm. ( B-C ) Sciatic nerve function scores and sciatic nerve indexes of mice treated as indicated. ( n = 10 mice per group; Kruskal-Wallis test with Dunn’s multiple comparisons test; data are shown as the means ± SD). ( C-G ) KPC mice (LSL-KRAS G12D/+ ; LSL-TP53 R172H / + ; PDX-1-CRE +/+ ) were divided into three groups. Mice were randomly assigned into three groups. Mice were injected with AAV-packaged shNC (AAV-shNC) ( n = 10) or with AAV-packaged shcircKLHL24 (AAV-shcircKLHL24) ( n = 10) into the tail vein at a dose of 7 × 10 11 viral genomes (vg) per mouse. The third group received intraperitoneal (i.p.) injection of anti-CXCL12 ( n = 10),40 mg/kg, once every three days. ( D ) Representative mIF images showing PNI status and CXCL12, Sec31A, FAP, expression in KPC mice treated as indicated. FAP served as a CAF marker. ( E ) Proportion of PNI in KPC mice under the specified treatments.(Fisher’s exact test). ( F-G ) Nerve density percentage (left) and number (right) in KPC mice following the indicated treatments. (Chi-square test). * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: KPC (LSL-KRAS G12D/+; LSL-TP53 R172H/+; PDX-1-CRE+/+) mice were purchased from Shanghai Model Organisms.

Techniques: In Vivo, Staining, Expressing, Marker, Injection

Journal: Cell reports

Article Title: Context-dependent inhibitory roles of RhoA in 3D invasive cell migration within the extracellular matrix

doi: 10.1016/j.celrep.2025.116649

Figure Lengend Snippet: (A) Histological analysis (H&E and Masson’s trichrome staining). Scale bar, 200 μm. The enlarged boxed regions highlight details. Scale bar, 100 μm. (B) PDAC progression stages ( n = 7–10 mice for each genotype). (C) Immunohistochemistry of pancreas stained for Ki67 and α-SMA. Scale bars, 20 μm for Ki67 and 100 μm α-SMA. (D) Quantification of Ki67-positive nuclei and α-SMA-positive area (mean ± SD, n = 4 mice for each genotype). (E) Representative pancreas images. Scale bar, 1 cm. (F) Pancreas weight quantification (mean ± SD, n = 6 WT, 7 KPC, 9 KPC::RHOA-KO mice). (G) Survival curve of KPC and KPC::RHOA-KO mice ( n = 10 mice for each genotype). (H) H&E-stained pancreas sections of KPC and KPC::RHOA-KO mice at 12 weeks post-tamoxifen injection. Scale bar, 1 cm. Tumor boundaries are outlined; boxed regions are enlarged. (I) Quantification of inverse circularity of tumors (mean ± SD, n = 20 sections from 6 KPC mice and 50 sections from 10 KPC::RHOA-KO mice). Statistical analysis: one-way ANOVA with post hoc Tukey (D and F), log rank Mantel-Cox test (G), and two-tailed Student’s t test (I): * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: KPC mice , Jackson Laboratory , Strain# 032429.

Techniques: Staining, Immunohistochemistry, Injection, Two Tailed Test